Antibiotic susceptibility patterns of propionibacterium acnes isolated from acne vulgaris in Assiut university hospitals, Egypt

Background: Antibiotics are frequently used to treat acne patients either as bactericidal or anti-inflammatory agents. However, with the increased use of antibiotics, resistant strains of Propionibacterium acnes began to emerge and have been associated with a poor treatment outcome

Objectives: Detection of staphylococcal and Propionibacterium acnes strains in cases of acne vulgaris in Assiut university hospitals, Egypt and antibiotic susceptibility patterns of Propionibacterium acnes isolates

Methodology: Microbiological samples were obtained from one hundred patients with inflammatory acne lesions. Samples were cultured on blood agar and mannitol salt agar media under aerobic conditions at 37[degree]c for isolation of staphylococcal strains, and on blood agar under anaerobic conditions at 37[degree]c for 3 to 7 days for isolation of Propionibacterium acnes. Bacteria were identified by colonial morphology, standard biochemical tests, and API 20A test for identification of Propionibacterium acnes isolates. Antibiotic sensitivity testing of Propionibacterium acnes strains was done against clindamycin, erythromycin, doxycyclin, trimethoprime/sulfamethxazole, tetracycline and levofloxacin

Results: Staphylocoocal strains were detected in 55% of acne cases, while Propionibacterium acnes were detected in 35% of cases. Most Propionibacterium acnes isolates were sensitive to levofloxacin [80%], followed by doxycycline [51.4%], tetracycline, trimethoprime/ sulfamethaxazole [20.0% for each] while showed highest resistance rates to clindamycin [85.7%] and erythromycin [82.9%]

Conclusion: Levofloxacin was the most effective antibiotic for Propionibacterium acnes followed by doxycycline, while Erythromycin and clindamycin were the least effective antibiotics for Propionibacterium acnes

Serotyping and antimicrobial resistance pattern of streptococcus pneumoniae strains in patients with community-acquired pneumonia

Community-acquired pneumonia is a common disease and a frequent cause of morbidity and mortality worldwide. Streptococcus pneumoniae is the most common cause of community-acquired pneumonia. The current study was conducted to determine the serotype distribution and antimicrobial susceptibility patterns of Streptococcus pneumoniae isolated from patients with community-acquired pneumonia at Assiut University Hospitals. From February 2013 to May 2013, sputum samples from 60 adult patients with community-acquired pneumonia were analyzed for bacterial etiology using conventional methods. Antimicrobial susceptibility and serotyping of Streptococcus pneumoniae was performed, bacterial agents were detected in 53 patients [88%]. Streptococcus pneumoniae was the most common [30%] isolated bacteria. Eight co-infections were identified. The detected pneumococcal-serotypes were in decreasing order; 1, 9V, 6B, 19F, 23F, 14, and 19A. Pneumococcal-antibiotic resistance was highest for penicillin and ampicillin antibiotics. Streptococcus pneumoniae is the most common isolated bacteria in cases of community-acquired pneumonia which is associated with certain serotypes. Resistance to penicillin and other antimicrobial agents increased rapidly during the last years among pneumococcal strains worldwide